Posted by chen1431 on April 22, 2008 12:38 AM|Permalink
Comments
One criticism for this paper is that there is no error associated with 'beta' or 'm'. A second criticism is that each point has a different degree of uncertainty.
1. What points have higher uncertainty in the y-axis, those at low or high concentrations of [ACBK]. Provide an explanation. [See figure 8 and equation 8].
Those points at high concentration of [ACBK] have higher uncertainty in the y-axis.In my opinion, the main uncertainty is the uncertainty of the dataImax. They assume that the dataI value obtained when the [ACBK] is extremely higher than [HSA] represents the dataI when all the HSA forms complex. However, it isn't ture since a very small free HSA remains at equilibrium. This uncerntainty is more significant when the difference of dataImax and dataI is small, that is, when the concentrantion of ACBK is high.
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Comments
One criticism for this paper is that there is no error associated with 'beta' or 'm'. A second criticism is that each point has a different degree of uncertainty.
1. What points have higher uncertainty in the y-axis, those at low or high concentrations of [ACBK]. Provide an explanation. [See figure 8 and equation 8].
Posted by: Edgar Arriaga | April 22, 2008 11:51 PM
Those points at high concentration of [ACBK] have higher uncertainty in the y-axis.In my opinion, the main uncertainty is the uncertainty of the dataImax. They assume that the dataI value obtained when the [ACBK] is extremely higher than [HSA] represents the dataI when all the HSA forms complex. However, it isn't ture since a very small free HSA remains at equilibrium. This uncerntainty is more significant when the difference of dataImax and dataI is small, that is, when the concentrantion of ACBK is high.
Posted by: Li Chen | April 24, 2008 11:36 PM