Posted by sato0041 on May 6, 2008 02:07 AM|Permalink
Comments
I really liked you presentation but I was curious about a couple things. First, and I'm sorry if I missed this explanation in class, how exactly is growth of the tumors slowed? (i.e. what's the reaction?) I understand how luciferase behaves as the reporter, but I didn't understand this point.
Secondly, do you know what types of cancer exhibit hMUC1?
As a post-presentation clarification note, I mention in my opnion of the summary that imaging mice at -105C is obviously not suitable to humans. After discussion today, this is also obviously a mute point as the CCD camera is cooled to -105C.
1) When the hMUC1 is incompletely glycosylated, a secondary structure of amino acids are exposed. If antibodies for this secondary structure have been created with vaccination/creation of antibodies, hMUC1 antibodies will bind to the hMUC1 and the cancer cell will be targeted for immune reponse. Once signaled for attack, the cells would be (hopefully) targeted for destruction like other foreign bugs and bacteria in your body. If the tumor proliferates faster than the immune system can destroy, however, the tumor will continue to grow.
2) "The MUC1 antigen is a self-protein overexpressed in carcinomas of the breast, ovary, lung, prostate, colon, and pancreas, among other carcinomas." Reference- Tang et. al., Blood, 104, 9, 2704 (2004).
sr means steradian.In bioluminescent image, the signal intensities were
expressed as photon flux (photons/s/cm2/steradian), where steradian (sr) refers to
the photons emitted from a unit solid angle of a sphere.
1. I noticed that FACS was used extensively as a validation technique. What is FACS? How was it used in this study?
FACS is Fluorescent-Activated Cell Sorting analysis. It was used to confirm that the hMUC protein was highly expressed in the CT26/hMUC1/Fluc cancer cells.
3. What defines the background noise when imaging chemiluminescence?
Dark Current, external lighting, and external fluorophores
The views and opinions expressed in this page are strictly those of the page author. The contents of this page have not been reviewed or approved by the University of Minnesota.
Comments
I really liked you presentation but I was curious about a couple things. First, and I'm sorry if I missed this explanation in class, how exactly is growth of the tumors slowed? (i.e. what's the reaction?) I understand how luciferase behaves as the reporter, but I didn't understand this point.
Secondly, do you know what types of cancer exhibit hMUC1?
Posted by: Anonymous | May 6, 2008 10:05 AM
As a post-presentation clarification note, I mention in my opnion of the summary that imaging mice at -105C is obviously not suitable to humans. After discussion today, this is also obviously a mute point as the CCD camera is cooled to -105C.
Posted by: Chad Satori | May 6, 2008 10:07 AM
I posted the last comment, but I think I forgot to put my name in. Just an FYI.
Posted by: Melissa Maurer-Jones | May 6, 2008 10:08 AM
Melissa,
1) When the hMUC1 is incompletely glycosylated, a secondary structure of amino acids are exposed. If antibodies for this secondary structure have been created with vaccination/creation of antibodies, hMUC1 antibodies will bind to the hMUC1 and the cancer cell will be targeted for immune reponse. Once signaled for attack, the cells would be (hopefully) targeted for destruction like other foreign bugs and bacteria in your body. If the tumor proliferates faster than the immune system can destroy, however, the tumor will continue to grow.
2) "The MUC1 antigen is a self-protein overexpressed in carcinomas of the breast, ovary, lung, prostate, colon, and pancreas, among other carcinomas." Reference- Tang et. al., Blood, 104, 9, 2704 (2004).
Hope this answers your question!
Posted by: Chad Satori | May 6, 2008 11:20 AM
1. I noticed that FACS was used extensively as a validation technique. What is FACS? How was it used in this study?
2. The bioluminescent signals were expressed as photons/cm2/s/sr. Explain what is sr?
3. What defines the background noise when imaging chemiluminescence?
Posted by: Edgar Arriaga | May 6, 2008 11:15 PM
sr means steradian.In bioluminescent image, the signal intensities were
expressed as photon flux (photons/s/cm2/steradian), where steradian (sr) refers to
the photons emitted from a unit solid angle of a sphere.
Posted by: Yu-Shen Lin | May 8, 2008 12:49 PM
1. I noticed that FACS was used extensively as a validation technique. What is FACS? How was it used in this study?
FACS is Fluorescent-Activated Cell Sorting analysis. It was used to confirm that the hMUC protein was highly expressed in the CT26/hMUC1/Fluc cancer cells.
3. What defines the background noise when imaging chemiluminescence?
Dark Current, external lighting, and external fluorophores
Posted by: Chad Satori | May 8, 2008 06:53 PM