Posted by olso4499 on May 7, 2008 02:52 PM|Permalink
Comments
1- I was looking at equation 1 in the paper, how do they determine the proportionality constant? Could you look at the supplementary material and explain to the rest of the class.
2- How could one determine the k and k-1 for the dual probe?
Eric, maybe I missed this during your presentation...but how does the ester-derivatized form of KCM-1 get converted back to the fluorescent form of KCM-1 inside the cell?
I have kind of a follow up to Nic's question, how efficient is the conversion from the ester derivative back to the fluorescent form? Does a high concentration of the ester derivative have to be loaded before you can see any appreciable signal?
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Comments
1- I was looking at equation 1 in the paper, how do they determine the proportionality constant? Could you look at the supplementary material and explain to the rest of the class.
2- How could one determine the k and k-1 for the dual probe?
Posted by: Edgar Arriaga | May 8, 2008 03:59 PM
Eric, maybe I missed this during your presentation...but how does the ester-derivatized form of KCM-1 get converted back to the fluorescent form of KCM-1 inside the cell?
Posted by: Nic Frost | May 9, 2008 02:18 PM
I have kind of a follow up to Nic's question, how efficient is the conversion from the ester derivative back to the fluorescent form? Does a high concentration of the ester derivative have to be loaded before you can see any appreciable signal?
Posted by: Craig Bishop | May 9, 2008 07:32 PM