ࡱ> qspa jbjbA]A] +?+? .......~  ~yyy,R t.yW"yyy..y..B$f....y.. CF0  .~~BD~~BProteomics Paper Summary Jon Dozier Reference: Tokarski, C. et al. Identification of Proteins in Renaissance Paintings by Proteomics, Analytical Chemistry, 2006, 78, 1494-1502. Bioanalysis and Sample: In this paper, the authors designed a methodology for determining the various proteins contained in the paint of Renaissance artwork. The authors use this methodology to look at what types of proteins are in Benedetto Bonfiglis triptych, The Virgin and Child, St. John the Baptist, St. Sebastianin and Niccolo di Pietro Gerinis painting The Virgin and Child. Importance: Currently art historians mainly rely on old manuscripts to determine how painters have changed their paints through the ages, this new technique allows them to accurately determine what types of mediums the painters used. More broadly, this technique could be use by archeologist or forensic examiners because it shows how to identify proteins from very old samples, which may have been exposed to many contaminants. This technique also provides a way for very small amounts of sample to be characterized using mass spectrometry. Technique: The proteomic technique described in this paper uses MS/MS to analyze the proteins found in Renaissance art. The technique involves first extracting the proteins from the complex binding mixture by grinding a sample of the painting with a commercial resin in aqueous solution that this 1% trifluoroacetic acid. A tryptic digest is then performed on the sample to break the proteins apart into smaller peptides. Nanochromatography is then used to separate the peptides based on their hydrophobicity. MS/MS is then used to identify the sequence of the peptides within the mixture. The technique uses ESI to generate the initial ions then quadrapole MS to get the initial molecular weight. After that radio frequency collision is used to fragment the peptides into smaller ions which are analyzed by time of flight MS. The computer programs ProID and Mascot are used to identify the proteins from the various MS fragments. Example: The results shown in figure 1 represent MS data collected after the initial peptide has been fragmented. From the results it is clear that this technique allows for the identification of peptide fragments very accurately. Even for a somewhat large peptide the authors were able to identify many of the peaks in the MS spectrum. Opinion: This papers shows that it is possible to identify very old proteins in very small quantities. 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