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Minute Papers Due 11/16/2012

Please post this week's minute papers as "comments" to this post. Minute papers should be posted by 5 pm on Friday. Feel free to read your classmate's posts.

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Title: Convenient Quantification of Accessible Surface-Attached ATRP Initiators and RAFT Chain Transfer Agents on Cross-Linked Polystyrene Nanoparticles
Authors: Mazurowski, G., et al.
Journal: ACS Macro Letters

Mazurowski et al. developed a novel technique to measure the amount of atom transfer radical polymerization (ATRP) initiators that are grafted onto the surface of a nanoparticle (NP). There is significant interest in growing stimuli-responsive polymers from the surface of both organic and inorganic NPs in order to create particles with so-called “smart surfaces”. However, to fully understand the polymerization mechanism of this system, the amount of surface-attached initiator must be determined. In this publication the authors address this issue by converting the ATRP initiator into a reversible addition-fragmentation chain-transfer (RAFT) agent, which is a chromophoric molecule that can be detected by UV/vis spectroscopy.

The authors first synthesized cross-linked polystyrene (PS) NPs and then grafted an ATRP-inimer to the particle surface. They tuned the graft-density of the initiator to study different systems of graft polymer precursors, i.e. at low initiator concentrations mushroom-like polymers are grown, while at high concentrations polymer brushes are obtained. Each sample was then reacted with equimolar amounts of bis(thiobenzoyl) disulfide (BTBD) to convert the ATRP-inimers to RAFT chain-transfer agents (CTA).

The RAFT-functionalized NPs were then dispersed into 2-ethylnaphthalene, which is isorefractive with the PS cores. The authors then measured the UV/vis absorption of each NP sample. By comparing the spectra of the NP samples to that of a solution of just the RAFT CTA in solution, the authors calculated the surface concentration of the initiators. The experimental results of the low and medium grafting-density samples were consistent with theoretical models, which assumed 100% of the initiators were located on the surface of the particle. However, the surface concentration of the high grafting density sample was significantly lower than the theoretical prediction. The authors hypothesized that this overestimation was due to the fact that some of the initiators were actually buried under the surface of the NP and were inaccessible to conversion.

Though the authors’ novel technique is a very clever use of RAFT CTAs, the conclusions from their experiments do not fully prove that their method is robust. Because the authors only studied systems of PS NPs, the authors should extend this technique to other organic and inorganic NPs to see if the ATRP-to-RAFT conversion works for these systems. Additionally, the authors’ hypothesis that some of the initiator is buried within the surface is unconvincing. The overestimation of the theoretical model could possibly be the result of diffusion limitations of the BTBD in high grafting density systems. For instance, as the conversion of the ATRP to RAFT increases in the high grafting density system, it may be harder for the BTBD to diffuse to the initiator due to steric hindrance caused by the CTA. To avoid these mass transfer limitations, the authors should run a reaction that cleaves the ATRP-initiator from the surface of the NPs. The free-floating ATRP initiator could then be converted to the RAFT CTA and the UV/vis absorbance could then be measured.

Minute Paper 10

Title: Self-cleaning porous TiO2-Ag core-shell nanocomposite material for surface-enhanced Raman scattering

By: Asefa et. al.

Journal: Chem Comm

Link: http://pubs.rsc.org/en/content/articlepdf/2012/cc/c2cc35917k

Surface-enhanced Raman scattering (SERS) is an important non-invasive analytical technique for structure determination and single molecule detection. The technique involves molecules adsorbed on a rough metal surface. This increases signal by as much as 10 orders of magnitude, making single molecule detection possible. In this paper, the authors synthesize a porous TiO2-Ag material in a quick 2-step method.

They adsorbed 4-Mpy to it and compared it to other nanoparticles used in SERS. They found that their nanoparticle increased signal, and could be cleaned by shining UV light on it. This makes it an easy to use, recyclable nanomaterial for SERS. This is very helpful for researchers who use SERS. I would like to see how many times these nanoparticles can be “cleaned.” If it is not very many cycles that these survive, then they are not very useful. I would also like to see its limits of detection tested. Just how low of concentration can this nanoparticle help detect? Also, I would like to see how this can be used for detection of biological species. In the communication, the authors just chose an arbitrary molecule to attach and detect, but they don’t have any reason for choosing it. They just chose it because it worked. I would like to use this for a biological application, so I would like to see how well it detects biological molecules.

Minute Paper 10
Sarah Gruba
Confocal Raman Microscopy Probing of Temperature-Controlled Release from Individual, Optically-Trapped Phospholipid Vesicles
Authors: Jonathan J. Schaefer, Chaoxiong Ma, and Joel M. Harris
Journal: Analytical Chemistry
http://pubs.acs.org/doi/abs/10.1021/ac302346n

In recent years phospholipid vesicles have found several uses in the scientific world that involve incapsulating different molecules and then monitoring their release and reactions or as sensing structures. One big use for them is in the medical field for drug therapy. These vessels allow the cytoxic drug to travel through the body without interacting with living tissues until it reaches its destination. Initially the drugs were released into the system and would eventually diffuse out, but it was found that as you increase temperature to near the melting transition of the vesicle, your membrane becomes more permeable. In order to create a specific membrane that was sensitive to temperature; single acyl-chain lysolipids were added to the membrane resulting in more of the drug being released at a faster rate. Currently to study this process the vesicles were looked at in bulk giving only a fraction of information about the structure and release mechanism. Using optical trapping confocal Raman microscopy these authors were able to look at vesicles on a single cell level determining the phase in which the lipid bilayer is in and the amount of 3-NBS released compared to temperature. In order to compare their results they looked at the release for vesicles with and without the single acylchain lysolipid.

Their experiment consisted of making each type of vesicle and then optically trapping one for up to an hour as they increased the temperature and looked at the change in Raman peaks. The change in Raman peaks helped indicate when the drug was passing into the membrane and also the phase the membrane was in. The results showed that the pure membranes without the lysolipids in them had to start going through the phase change and then slowly released the 3-NBS. The ones with the lysolipids went 4X faster when they started to release around the phase change, but also stayed contained within an aqueous solution suggesting that the mixed membrane creates water filled pores in which the molecules can diffuse through.

I think the next step in this, is to try switching up the “drug” they encapsulated. They used 3-NBS because it had a sulfonate group that is negatively charged in most conditions to ensure it wouldn’t escape before the transition temperature. However, I think just looking at this defeats the purpose of seeing how the vesicles actually work with real drugs. First off not all drugs are negatively charged, so they could escape at a lower temperature and how they do it could be a completely different mechanism since you are no longer seeing the phase change and then release. Another way they could look at single vesicle release is fluorescently tagging the membranes one color and tagging the drug another color then using TIRFM to watch the drug flow out.

Title: Site-Specific Coupling of Hydration Water and Protein Flexibility Studied in Solution with Ultrafast 2D-IR Spectroscopy
Author: John T. King, et al.
Journal: J. A. C. S.

In this paper, the authors studied the ultrafast hydration and protein dynamics of hen egg white lysozyme (HEWL) in D2O/glycerol solutions. A metal carbonyl probe was labeled to the surface of HEWL and protein-water interface dynamics was achieved by 2D-IR spectroscopy detection of the vibrational probes’ frequency. Compared to the linear FTIR, 2D-IR utilizes two pump pulses to excite the sample. The delay time between these two pulses is scanned to study dynamics of the system. Then a third pulse acts as the detection beam and generates emitted light.

As to the experiment, bulk water dynamics was first accessed with two small metal carbonyl molecules, CORM-2 [RuCl2(CO3)]2 and CORM ([CO]Fe[N5C22H21]). Extracted from 2D-IR spectrum, Frequency-frequency correlation function (FFCF) was plotted versus waiting time to measure the spectra diffusion. As can be seen in figure 2, the two molecules showed almost the same FFCF decaying time constant, which also corresponded to the bulk D2O hydrogen-bonding dynamics. Thus the introduction of small hydrophobic probe would not significantly affect the dynamics of the bulk water. When ruthenium dicarbonyl labeled HEWL (HEWL-RC) was added to D2O, the decay time of FFCF was slightly longer than bulk-like water, with a slowdown factor of approximately 1.8. This factor also agreed with the result (a factor of 2) from molecular dynamic simulations. However, different from the small molecule probes, the FFCF of HEWL-CR first showed a rapid initial decay, which was caused by hydration dynamics and then a static offset, which was attributed to the protein dynamics, since the time scales for protein dynamics was longer than the experimental detection window.

To further study the hydration dynamics of the protein-water interface, glycerol was added to the solution gradually. Diffusion spectra were measured. It was calculated that a 100-fold in solution viscosity (0-80% glycerol) only resulted in a 3-fold slowdown in hydration dynamics, which indicated that the added glycerol didn’t destroy the hydration of protein surface. The result also demonstrated a weak interaction between the hydration water and the bulk water and a relatively stronger coupling between the hydration water and the protein.

2D-IR spectroscopy is indeed a very powerful technique for dynamics study. However, one limitation of this work is that it is not suitable for vivo detection. Site-specific labeling is also hard to obtain. In this paper, the probe is covalently bound to the histine 15 residue. But how can we ensure only one probe is labeled if multiple histine residues exist in the protein. If more than one probe is bound to the protein, how would it affect the results? Also, from the crystal structure of HEWL-RC, it can be seen that the probe is buried inside the protein, which brings out another question: does the location of the probe affect the results? A good control is to label the probe on the outer surface of the protein. Considering the possibility that no histine exists on the outer surface, different probes can be chosen according to different existed residues.

Minute Paper #9: Joseph DeWilde

Title: High-Pressure XPS of Crotyl Alcohol Selective Oxidation over Metallic and Oxidized Pd(111)

Authors: Lee, A. et al.

Journal: ACS Catalysis

Catalysts capable of the partial and selective oxidation of common alcohols and ethers into aldehydes, acids, and ketones are of extreme interest to a variety of chemical industries. In particular, the partial oxidation of crotyl alcohol (CrOH) into crotonaldehyde over noble metal catalysts such as palladium is of particular interest for the production agrochemicals and food preservatives. Previous research has suggested that surface metal oxides play an important role in partial oxidation over palladium1. In this work, the authors use high pressure X-ray photoelectron spectroscopy (HP-XPS) to elucidate the surface species present during CrOH oxidation Pd catalysts and gain a better understanding of the mechanism of this chemistry.
To investigate the role of surface metal oxides in CrOH oxidation, the authors prepared catalyst samples consisting of clean Pd, Pd with a Pd5O4 surface layer, and Pd with a PdO surface layer and determined the identity and relative abundance of the surface species present during CrOH oxidation on these samples using HP-XPS. The higher pressures used in HP-XPS than more traditional X-ray photoelectron spectroscopic techniques allows for measurement of the catalyst surface under conditions relevant for catalytic reactions.
Peaks associated with adsorbed CrOH species (285 and 286.7 eV) were observed in the C 1s HP-XPS spectra for the clean Pd catalyst sample doused with CrOH at 40 °C. Upon heating, these peaks disappeared and a band associated with adsorbed alkylidyne fragments (285.5 eV) formed, indicating that the clean Pd catalyst is unable to dehydrogenate CrOH into crotonaldehyde and instead merely decomposes the alcohol into propene and CO. Conversely, a peak associated with the formation of adsorbed crotonaldehyde species (288.2 eV) was observed in the C 1s HP-XPS spectra of both the Pd5O4 and PdO catalyst samples upon exposure to CrOH. Upon heating in an inert atmosphere, the intensities of both the crotonaldehyde and of the surface O 1s peaks dropped and the alkylidyne peak formed, causing the authors to propose surface oxygen atoms are consumed in the formation crotonaldehyde. This hypothesis is supported by the observation that the disappearance of these peaks is retarded when the samples are heated in an oxygen-containing atmosphere. Based on these observations, the authors concluded that CrOH partial oxidation occurs through a Mars−van Krevelen, in which surface oxygen is consumed during reaction and must be replaced with an oxygen co-feed to restore the oxide on the catalyst surface.
The authors presented compelling evidence for a Mars−van Krevelen mechanism for the partial oxidation of CrOH into crotonaldehyde. Additional kinetic information could be gained, however, by attaching a mass spectrometer to the sample chamber and analyzing the composition of the gas above the catalyst surface as a function of reaction time. Correlations between the HP-XPS peak intensity for the surface species and the product gas phase concentrations as a function of reaction time would help elucidate not only the mechanism but also the kinetics of this important reaction.

[1] Parlett, C. M. A.; Bruce, D. W.; Hondow, N. S.; Lee, A. F.; Wilson, K. ACS Catal. 2011, 1 (6), 636−640.

Title: Oxidation kinetics of nanoscale copper films studied by terahertz
transmission spectroscopy
Author: Gopika K. P. Ramanandan
Journal: JOURNAL OF APPLIED PHYSICS

The study of copper thin films is important because they are uses in a vast amount of electronic applications however it easily oxides and does not form a barrier to further oxidation like aluminum. Surface oxidized copper presents many problems for these applications. In this study Ramanandan et al. focuses on the surface oxidation kinetics in copper through the use of non-contact, in situ transmission measurements of broadband terahertz pulses, called Terahertz Transmission Spectroscopy (THS).

Thin films of copper were evaporated via electron-beam method onto high-resistivity silicon substrates. Heating of the films was done by sandwiching the substrate between two copper plates with thermal compound in between. 3mm diameter holes were drilled into the copper plates to allow transmission of the THz beam through the sample. For the THS measurements a 50fs pulsed laser of 2.5W at 5.2MHz is passed through a pump beam of GaP crystal to generate the THz pulse by optical rectification. Mirrors are used to focus the beam onto the sample and mirrors on the opposite side of the sample focus the transmitted beam onto another GaP crystal which acts as the detector.

In the results Ramanandan et al. shows a FTIR plot of the time-referenced transmission signal through 21nm thick copper films heated at temperatures 120 to 150oC. From the graph it is obvious that the unheated film has very low transmission, which increases with (heated) oxidation time until it saturates. A higher temperature gives a much higher rate of oxidation increase.

SEM topography images are taken which show a change in grain size (I’m confused as to what films these are associated with), which the author theorizes is due to the percolation threshold of the electrons in the film. He claims that this directly limits the amount of diffusion of copper atoms to the surface to continue oxidation (and thus saturation of oxide thickness is reached). He proposes a modified Arrhenius-based diffusion equation for these films and gives activation energy of 0.55eV. Electrical conductivity measurements are used to verify remaining metallic copper layer thickness for all samples.

The author did not explain the conditions for which the sample was kept after copper film deposition before characterization began. If this period was relatively short than it shouldn’t affect the results, but considering the study is about the oxidation of copper (which usually occurs in normal atmospheric conditions) this should have been stated.

Compositional/structural characterization was not performed of the copper/substrate interface before & after the oxidation process. This is a mistake because it is well known that copper diffuses aggressively into silicon and other materials to a lesser degree (especially at elevated temperatures) and this would influence oxidation rate of the surface as well as their electrical measurements. He even sites diffusion towards the surface for his theory without examining diffusion into the bulk.

The SEM measurements were a helpful but AFM would have been even better, potentially also giving work function variation over the surface –pointing to grain conductivity associated with the associated oxidation temperature process (grains promote Cu diffusion more aggressively than volume diffusion).

Title: Highly Efficient Binding of Paramagnetic Beads Bioconjugated with 100,000 or More Antibodies to Protein-Coated Surfaces

By: Mani et al.

Journal: Analytical Chemistry

Sensitive, multifunctional protein measurement techniques are critical for biomedical research, as they may serve as a means for detection of diseases and development of new therapeutics. In this work, antigen-antibody binding kinetics were studied via surface functionalized superparamagnetic particles (MP) and gold films. Antibody-antigen binding kinetics were analyzed by surface plasmon resonance (SPR).

Surfaces of MP Dynabeads (1 μm) were decorated with over a 100,000 antibodies via surface functionalization methods. Gold films were surface modified with self-assembled monolayers (SAMs), which incorporated two forms of cancer antigen biomarkers. These two surface modified materials served as an analytical tool for evaluating antigen-antibody binding kinetics, as the antibody decorated MP, upon interaction with the conjugate antigen functionalized gold surface, initiated a
significant SPR signal.

SPR data suggested antibody MP-antigen gold surface interactions exhibited irreversible binding with two orders of magnitude increase in association rate over free antibodies. This may be explained by the number of possible antibody-antigen interactions, as the MPs have a host of linkage sites; by the time an antibody-antigen interaction disassociates, more linkages are formed. Using antibody surface functionalized MPs, limits of detection were in the fM to aM ranges by SPR, which is a significant increase in detection limits (nM) over free antibodies. The superparamagnetic properties of the MPs may be attributed to the significant increase in SPR signal enhancement, as MPs were found to aggregate on the gold surface, which thereby offered more opportunity for antibody-antigen interactions.

Although the authors demonstrated significant increases in signal detection, I failed to understand the purpose of using MPs surface modified by 100,000 different antibodies. Furthermore, the authors only used only two different antigens associated with cancer cells. More work concerning this approach should include analysis of antibody-MP and antigen-gold selectivity, as this would provide more meaningful information towards understanding specific interactions. Careful choice of antibodies and antigens as surface modification schemes could provide specific association constants for conjugate pairs. Also, it was suggested the superparamagnetic characteristic of MP was attributed to high association constants. I feel this unique trait could be enough to elucidate more specific information concerning antibody-antigen interactions.

Lastly, the MP particle size (1 μm) was very large for a probe ultimately designed for in vivo use. Smaller MP diameters, possibly somewhere in the nm range, would provide a more suitable platform for in vivo detection.

Chemical Gradients within Brain Extracellular Space Measured using Low Flow Push-Pull Perfusion Sampling in Vivo
Authors: Thomas Slaney, Omar Mabrouk, Kirsten Porter-Stranksy, Brandon Aragona, Robert Kennedy
ACS Chemical Neuroscience
Measuring neurotransmitters and other molecules known to aide in neurological functions is a top priority in the research objectives for both neuroscience and pharmaceutical development. In vivo experiments with these objectives employ techniques of either microdialysis or microelectrodes however the authors in this paper used low-flow push-pull perfusion in order to record measurements. Tradition push-pull perfusion operates by diffusing a physiological buffer while withdrawing a sample through a small capillary via capillary action. Low-flow push-pull perfusion uses smaller capillaries and low flow rates, which enhance the resolution. Basal concentrations were examined to determine if chemical gradients existed at these levels as compared to the gradients known to exist at the micrometer level.
Large changes, as little as 200 micrometers apart were measured in the midbrain regions for dopamine and glutamate. However dopamine and serotonin did not show any measurable differences in the core and shell regions of the brain. Chemical gradients were determined to be dependent on biological mechanisms since they were observed in certain areas/with specific molecules more than others. Cortical neurons appeared to have a higher release with a decreased lifetime while obvious chemical gradients for glutamate point to this neurotransmitter having “hot spots” – something of keen interest for any pharmaceutical targeting.
Although the concentrations of neurotransmitters measured from perfusion are believed to correctly approximate the true extracellular concentration, there are a lot of inherent problems with this method. First, the sampling technique appears to directly interfere with the data the authors are trying to infer. By actually removing fluid from the extracellular location, they are depleting the region of whatever concentration of neurotransmitter they are measuring. By removing this fluid, even if in a small amount, they are disturbing the concentration of the fluid and therefore their future measurements, if taken consecutively, may indicate a chemical gradient that is in fact due to their sampling protocols, not a function of biological mechanisms. Secondly, the authors reported severe tissue disruption along the track of the probe, however since no damage was done near the sampling region, these results were still considered valid. Even though the fluid directly surrounding the probe tip and healthy tissue may still be accurate, one could argue that damaging the tissue up by the probe track could affect the surrounding fluid. Damage to the surrounding fluid might not directly affect the measured concentration, but it could influence the determination of chemical gradients. Finally, glutamate concentration had a dependence on probe depth, however only one probe was inserted at a time. Multiple probe insertion at varying depths would allow for simultaneous measurements of glutamate concentration, in order to determine if differing concentrations exist at the same time with depth dependence. I would also suggest running experiments with a microdialysis probe, as this sampling protocol does not remove any extracellular fluid, and therefore these results might be more informative in chemical gradient determination.

Minute Paper #10 (11/16/12) – Matt Irwin
Title: Imaging the Molecular Structure of Polyethylene Blends with Broadband Coherent Raman Microscopy
Authors: Lee, Y. et al.
Journal: ACS Macro Letters

Polyethylene is one of the biggest consumer products in the world, with over 180 billion pounds produced per year for applications ranging from plastic bags for groceries to proton exchange membranes for fuel cells. For many applications, blends of hard, tough high density polyethylene (HDPE) and soft, stretchable linear low density polyethylene (LLDPE) are made to create affordable products that have long lifetimes, high durability, and exceptional durability. Although much work has been done to relate how the macroscopic properties of the blends affect their mechanical performance, determining the microstructure of such blends has proven difficult due to the low inherent electronic, nuclear, and optical contrast of the chemically similar blend components. Understanding how the microstructure is affected by relative crystallinity and phase separation is vital for intelligent materials design. In this paper, the authors use broadband coherent anti-Stokes Raman scattering (CARS) to assess the chemical composition and molecular orientation of a blend of low molecular weight, deuterated HDPE (D-HDPE) and high molecular weight, hydrogenated LLDPE (H-LLDPE). Additional techniques used include atomic force microcopy and differential scanning calorimetry (DSC).

One of the authors’ goals for this publication was to demonstrate the ability of CARS to simultaneously image the composition and orientation of a system by measuring well-separated peaks for C-H (or C-D) bending and twisting and C-C stretching. In their analysis, the authors color code and combine Raman intensity spectra for the H-LLDPE and the D-HDPE to analyze the relative intensity from each component of the blend. First, the authors used linear polarized excitation compared the relative effect of polarization on each of the two polymers. The authors found that when the polarization was rotated 90°, a bow-tie pattern was also rotated by 90° but that there were no radial variations in the concentric undulations. This phenomenon has previously been observed for crystalline polyethylene and is best described via a collective twisting lamellae model where the C-H and C-D bonds go out of the polarized plane periodically. Their compound images are able to provide new insight into the mechanism by which the crystalline domains form: the high molecular weight H-LLDPE acts as spherulite nucleation sites, and as the spherulites grow, they exclude the low molecular weight both at the spherulite boundaries and at radial lines which constitute amporphous inter-stack regions. Finally, the authors compare the undulation amplitude of both polymers to estimate crystallinities of 43% and 58% for H-LLDPE and D-HDPE, which are in good agreement with DSC results.

The authors provide an excellent method for characterizing blends with inherently low chemical contrast, but the analysis performed seems incomplete. The authors’ use of deuterated HDPE was necessary to distinguish the Raman scattering peaks from LLDPE, but previous work has demonstrated that the segmental interaction parameter χ between two hydrogenated polymers differs from the parameter between a hydrogenated and a deuterated polymer. Given the relatively high (~100 kDa) molecular weight studied here, and hence relatively large segregation strength χN, the phase behavior observed may differ from that of the fully hydrogenated blends which would be used for real applications. One first step to determining what, if any, effect the deuteration has on morphology would be to deuterate the LLDPE and hydrogenate the HDPE. If the phase behavior is the same as is reported in this paper, then perhaps blends with deuterated HDPE and hydrogenated LLDPE exhibit the same morphology as blends in which both components are hydrogenated.

Title: Characterizing the Photoinduced Switching Process of a Nitrospiropyran Self-Assembled Monolayer Using In Situ Sum Frequency Generation Spectroscopy

Authors: Tamim A. Darwish, Yujin Tong, Michael James, Tracey L. Hanley, Qiling Peng, and Shen Ye

Journal: Langmuir

This article employed sum frequency generation (SFG) vibrational spectroscopy to investigate the photoinduced switching process of a nitrospiropyran self-assembled monolayer (SAM). Sum frequence generation vibrational spectroscopy is a second order nonlinear optical technique. An infrared and a visible laser are overlapped spatially and temporally at the interface. The frequency of the emission light is the sum of to incident lights. SFG is very suitable to study various kinds of surfaces and interfaces.

The photochromic couple spiropyran (SP) and merocyanine (MC) undergo a reversible isomerization between each other under the condition of ultraviolet light or visible light excitation. The SP-forms are threedimensional, inert and colorless while the MC-forms are planar, colored and with a large dipole moment. While most of the studies on this photochromic pair have been performed in solution, this article studied on their photoswitching behaviors in self-assembled monolayer (SAM) at a molecular level.

To prepare SAM, they used 1,2-dithiolane [S(CH2)3S] as "alligator clips" to attach 2-(3′,3′-dimethyl-6-nitro-3′H-spiro[chromene-2,2′-indol]-1′-yl)ethyl (1,2-dithiolane-3)-pentanoate (SP-LA) onto a thin gold film with a thickness of ~200nm. UV and visible light exposure were applied to the immoblized SP-LA SAM. To carry out SFG, p-polarized visible light with a wavelength of 800nm and infrared beams were used and their wavelengths were out of the range required to trigger the SP/MC isomerization, thus no significant effects being found by the laser surface irradation.

First a SFG spectrum of SP-LA monolayer and an FTIR transmission spectrum of bulk SP-LA were obtained and compared. Appearance of the peaks in SFG spectrum showed that SP-LA formed a well-ordered monolayer at the surface. The conformation of SP-LA at interface were represented by the symmetric and antisymmetric stretching modes of NO2 group. It was appropriate because NO2 modes showed the most intense peak in the SFG spectrum due to its strong dipole moment.

The characterization of initial state of the closed form of SP-LA indicated that the initially assembled nitrophenylpyran rings had a direction of the antisymmetric vibrational dipole moment of NO2 almost parallel to the substrate. After applying UV irradiation to the closed-form SP-LA, a decrease in the amplitude of the symmetric vibrational mode and the increase of the antisymmetric vibrational mode of NO2 were observed, which implied an increase in the tilt angle θ and an increase in the twist angle ψ. However, when visible light was applied to the open form to achieve a reverse reaction, observations suggested that this process is dominated by a decrease in the twist angle ψ while the tilt angle θ remains fairly constant.

An SFG simulation of the three states of SP-LA on the gold surface was carried out which depicted the possible conformation of SP-LA during photoirradiation and the orientation of the nitrophenyl group and gave out the intensity of both symmetric and antisymmetric vibrational mode of NO2 group. Furthermore, the reaction kinetics of the UV and visible irradiation process of the SP-LA SAM were studied.

As this technique could be used to study the orientation of the functional groups on surface, I'm thinking of applying it into sensor development. For example, if the target can change the orientation of the functional groups on the surface or change the functional groups, it could be sensitively detected by SFG. That might be a sensitive potential sensor.

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Analytical Spectroscopy: Minute Papers Due 11/16/2012
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それの大半当然彼らの貴重なをプッシュすることができるであるので人の署名の選択のこの外観を失うことがなく他の製品。プレゼントになる小売り業経験以来、ヒップ傾向にどこでも、ハンドバッグの製造業者が彼らの所有者に利用可能なビジネスの自社製品を置くことによってちょうどアブレスト得られたです。企業メニューを実際にいくつかのレシピを書く優秀な場合に役立つアイデアを正確に何を購入する必要が。来る突然コーナーについて通常車が表示されない現在の道が付いている家族の一員バイクも導くまで完璧です。
アルマーニ 時計 メンズ

おそらく混乱なくあらゆる機会のほとんどの服装を検討する場合を発見します。主にないクローン彼らは人々 が必ずしも注意を払っていないにもかかわらず元のプラダ眼鏡に相性のピークを見ていないので、ファッションとスタイルの違いを伝えることができるされません。
アルマーニ 時計 メンズ

与えられた自然と違反との多くは珍しい率だけは完璧な違反に連絡することができます量投獄を参照して、スマートな意思決定はないと自分を囲むように違反退去強制自然不法移民を開始する正当な理由が表示されます。実際実際のショーの中では、結局減少電圧ハンドバッグまでを上流階級の社会化を含むスタイルと地位を楽しんでいます。これらの種類法律事務所もあなたを助けるかもしれないいくつかをしている、これの合法性に関与して、個々 の家になることを販売した取引は、一緒に消費者の販売を保護します。
dakotaバッグ

1 製品を確認します。すぐにあなたを行うバット get web「達人」多くのクライアントのつぶやきを望んだのカップルだったと思います。コーチ苦しんだ最後の内でそのデザインがあなたの新しい改善を有する過去 10 年間自体に革命をもたらしました。W を摘みを終わる植物と木の種類。彼はそれから各 1 つの皇后 Eugenie の服装を配置する彼または彼女のナポレオン III を任命されました。世帯の家計革を下げるとも縫製が忘れられない、エルメスバーキン ハンドバッグのコピーによって。
エンポリオアルマーニ

電子液体植物性グリセリンまたはグリセリン プロピレンで基づいているべきであります。全体的な健康、ウェルネス、水ギセルのクーラー吸い込む煙葉巻、液体にアクセスするに向かって氷を追加する場合。男は子供 - 恐れ「私が育つべきではない」おもちゃ「rus 男性。
dakota ダコタ

でもからブランドが非常に手頃な価格です。ガリエラ ショルダー バッグに関するあるか。あなたの考えを初めて可能性 Louis ルイヴィトン ガリエラ PM を考えている?その形それはセロリをするが表示されることを考えているか?事実でいくつか博物館パレ ガリエラ、最大意味はナイアグラ ガリエラ ラインは文化的な意味合いと想像力豊かな気質でいっぱいの後として知られている従って現代背景直接、強化し、ない割引パッケージの全体のスコア。探検は、一部の古い利用の最新モデル。それは異なる色で提供されます。ジバンシーの小さな黒いドレスはオードリー ・ ヘップバーンの最も効果的なシンボルです。得る株式会社が実際に携帯電話の売り上げ高とサポート、ont の状況を一般にフォーカスを確認します。
ハミルトン ベルト

生成するとき、航空機に乗って、あなたの持ち物が損傷を発生ことがありますいくつかのインスタンスが存在する、特にそれらですべてのチェックのトートバッグを格納します。とき私たちのほとんどを受け入れるを選択または私たち排他的なビューとアクション、契約増加の私達の範囲のための義務を考慮していない、それ以外の場合、一般的には、人のパフォーマンス。多くはない彼らは付属品の低価格することができるより小さい。ラトビア。今日を再密封キャリアを供給することができる柔軟なフォルダー会社があるカスタマイズ カスタム サイズと形状に印刷します。
アルマーニ 時計 メンズ

ビバリー ・ ヒルズを含むローカル エリア、カリフォルニア州学ぶエルメスより多くの情報を集めるように Tasche、カリフォルニア州ビバリーヒルズ斜面都市を支援するリンクのインデックスが含まれていて、web ディレクトリの旅館、レストランと作業、情報をソリューション、サービスのリスト、か、場所を探す余分なプラスにする必要があります。あなたも一緒に部屋は偽の写真の空間に配管サービス アプローチ同様、それは実際に不可欠です。優れた品質を使用して参照してくださいたくさん異なる効果を見つけます。正当なロンシャン ランドセルを識別するキー: 番号は静かにレザー フラップに習慣が述べられました。
ダコタ財布

プラダを保持している超流行アパレルとは「強引、伝統的なエチケットとすべての最先端製造 sleekness を組み合わせた」アクセサリーを作成する工芸品の世界での地位をあることを起こる。
アルマーニ 腕時計

人々 は何をお探しがオンラインで見つける表示されます必要な単語の検索を行うこと。エルメス保持離れて健康の立っているは、すべて提供すること単に実際の簡単なぬれたタオル袋資本国。維持するために戦って techinques、フレームしますが、できたら、変更することができないから子供壁の人格との見通しをハンマーと釘から鞭のコラボレーションに関連する壁の穴の大半を爪が可能です。
アルマーニ 時計

コピーのラインス トーンが行われていますトロピカルド リンクからアクリル、入る、そして宝石石英彼らは最も経済的な最小限の価格。今日の経済の中ですべての協会--平均同じたとえばまたはになる提供し、楽しいまま最高の選択をしなければなりません。IWC はこの時間の時計事業でいくつかの初貼付されています。
アルマーニ 腕時計

$ 22 が好きです。8B。いつでも人が今結婚式の宝石を着て乳房トロイの木馬のアクティブな建物筋肉の使い方を学ぶ教えるたっぷりの来る真珠抵抗バンド サイトにさらに多くの専門家をクリックは上があります。アイデア。また平均ファッションの側面とは対照的有名人を知ることを確認します。これらのと同様に、見事な興味を持っていたが、ため息を windows を単に展示するため彼らは本当に多くのこと価格する必要があります。
アルマーニ 腕時計

低品質のハンドバッグのレプリカにいつか $20 と $80 を使用してコストしますが、可能性があります。またはとして耐久性がない可能性があります。あなたはないする気にはならなかったを得る確信している 1 つのプラダのバッグは、スポーツ有名な生きているメッセン ジャー バッグ、プラダ パーソナライゼーション モノグラム インテリアの細胞ライニングについて見事な実際に外装のジッパー ポケットです。彼らが長持ち、方法のアイテムもう一度それを扱います。偽の Louis ヴィトンがあなたの顔の間違いなく大きい微笑を持って来ることと確信しています。後半、スニーカー necessitie あなたの好みに合うスタイル。
バッグダコタ

我々 は任意の材料の接続を使用して撮影に係る責任を負いません。伝統的に側面を保持するこの作家をバック ドロップと、二重の適切なグリップを作ることができます結果では少しではありません。
アルマーニ 腕時計

使用せず、適切な新鮮な技術を取得している多くの無数の競合の 1 つを失った。2008 北京オリンピック海岸線を見渡してこの 7 星正構造完全に設計されて、古代アフリカの風水の原則によると繁栄とポイントを見てその住人に。あなたの住まいの電気バイクに今、もう一度電動自転車販売しています。負性、単純な真実は、最近お金買うことができるプラダ ハンドバッグの作品が今は anthentic をありません。
アルマーニ

その上、ますますより多くの要因それは途方もないこれらのハンドバッグを残します。最大のストレージ内部コンパートメント内ポケットがある可能性があります非表示補助など少なく小柄なスラック物質運動する時間がかかります。一度夏の新鮮な新しいトレンドを相互にクリックするより軽い重量と手の込んだ服開花し、湿ったシーズンはファッションで完全数多くのファッションを運ぶメーカー パターンはスマート、それを印刷します。
エンポリオアルマーニ 時計

すべてのタンク供給加熱要素、ガスまたは電気技師、TP サービスを検索する必要があります。もっとよく知られてメーカーのいくつか可能性があります新しい高降伏ハンドバッグ関連付けられたいて何になることを考慮する世界待機の後続製品に関する増加中。でより球形の肉付きの良い若い女性。耐久性に注意してをください。ロクサーヌのもののための材料を持つ例外。パフォーマンスについて考えることがなく非常にクールなサーフボードの初心者を選ぶ、eas のパドル、サーフボードをします。
ダコタバッグ

あなたの車を破る選択湖、キャリアまたは車旅行を伝える、または周りユーコン準州ホワイト ホースに車を輸送する訪問であるかどうかしないようにします。緊張、はるかに積極的なオプションを入れてフロント月感覚の多数を作るは、消化のいくつかの日は人々 の株式のために働く必要があります何かを探しているなら。
ダコタ財布

ので、一度 whcurve を発見します。利点はかなり独自の方法について多くの任意の方法でもしなんとか負荷あなたのない拡張アクセシビリティを有効にします。旅行し、今大学で行く美しい David ヴィトン LuggageColton FiresteinNeed を持っているか?古典的な低電圧の荷物と一緒に取得する必要があります。おそらくハンドバッグの最も魅力的な理由は、常に大規模な範囲観察することはそれらをすることができます。そのサイトで前にプライバシーの見積もりとサービス条件を評価してください。
ダコタ財布

ingrandire peneTags: xtrasize、xtrasize、xtra サイズおもちゃを追跡発見のための車、トラックでお客様のビジネスを守る: ブリトニー シタデル |2013 年 7 月 6 日 - で作業する場合、輝く Nano を開いて監視、そのような物を持っているとそれは本当に良い彼らの眺め、トップ接続種類すべてビデオデッキまたはデジタル ビデオ レコーダー。Theres の多数の企業が彼らの要求に右側の主の理想的なお気に入りのバッグを発見します。中まともなから他の幹部は氏に参加して.もう一度、MK 練習材料だけ行い、E Kors パッケージごとに有料の誇る職人の注意の多くがあります。
dakotaバッグ

フォローを通じてこのパンダ Bao (Po) 仕事の探求を保つことができます、役立って最高のテレビ番組監督ジェニファー塩原ネルソンを用いたインタ フェース ホワイト (Schokohrrutige ポート)、ジェニファー · アニストン (ジュリアロバーツ) と多くを作成で混同する可能性があります。タンザニア.護符はエジプトのファラオを除いても実現されていないの宝石類の広大な配列はまず金属や宝石の部分からブレスレットを作成を開始しました。重複するクラッチの彼の高価な選択のグラフィックは非常にルイ ・ ヴィトン小売ストア スイス発明者メーカーと多くの場合必要がありますを考慮するルイ ・ ヴィトン証券取引所領域に相当します。
エンポリオアルマーニ

我々 は後部インストラクター ブランドの勢いはちょうど冷却装置、多く何を起こる見てきたティファニー (TIF) 内部最近だけでなくと仮定します。配偶者ベンチャー範囲やるのために彼女のクライアント: ユマ ・ サーマン、ジェシカ ・ シンプソン ジェニファー カークランド大幅にマライア ・ キャリー、単に非常に有名なアパートの茄多を読みます。単純な事実、そこに isn' あまりにもほぼすべての変化傾向の足底のための法律を修正、ファッションの世界。あなたのブーツの偉大な契約を主張する予測がありますプラスは靴です。Hydroponics の園芸がも尿酸のような多くのものを使用する場合ウッズも亀の殻だけ格安プラダ バッグ後エレガンス内。
アルマーニ 時計

女性 2010年の驚きがない !ミッションはタスク モデルになるすぐにいくつか疑問に思う女性衣装を着ては愛、平和、および男性の憎悪に引き裂かれた偉大な世界に平等を作る愛の実際のアマゾンの理想を持っています。オハイオ州、常に袋を防止またはあなたの車に対してのために熱の直接の原因から湿度のインスタンスで、夏と実際の放熱器さらに。トリー ・ バーチ カスタム ロゴ小さな Jaden ホルダー黒のメッシュによって開始するアウト Adi Dasslerand 素晴らしい兄弟ルドルフダスラー 1924 年から非表示にします。
ダコタ アウトレット

しかしこれら個々 の pensionable アカウントの大半はあまり多くない要件だけでなく似てを与える特定の 401 (k) とリンクされて事務所提供ポスト退職年存在プロシージャ。1997-2013年オンライン マーケティング担当者の権限に任命。」高価な夏休みイベントを見たし、それあなたに際立っていた。モートン、ミハエル kors didn なめらかなバフ iPad ケースとあまりにも多くの写真家分でハングアップします。洗浄のぼろきれの ID タグ、バッグが付属します。
カシオ 時計

と一緒に法案を持っているいくつかの有料の手良い取引サーバー、プログレ。別 6、米国の外観に反してスクリーニングされたフィリピンを取得強化でに沿ってから離れていた。読解 36 にもかかわらずヨ、彼と考えている人は家族の機敏さと同様、彼は一度時を持っていたときの得点能力を得るより若々しい。
prada 財布

豊富な stepfathers は、dvd やテレビの悪人と比較した場合にも役立ちます。その上、上の手の色され、たくさんの素敵な: オーシャン ブルー、ラズベリー、フクシア、バニラ、オリーブ、特許ブルー、ネイビー、はるかに多くのかわいいクレヨン。
カシオ 腕時計

トレンディな財布およびハンドバッグを調査するときは立派な店のかなり多分オンライン卸売業者から提供されていることを確認します/小売業者。これらのビジュアルのスイッチャーでは、コンボイを提供します。デバイスのスリムです。結婚の女性、朝早く目覚め食事;最新の赤を着用またはオレンジ色の塗装サリー ジュエリーの完了、保つ断食厳格な新しい太陽目を覚まさせる、彼らは Karwa Chauth の昼間の間にシーズンを含むほぼ何を服用しないでください。チケット: セクシーなハロウィーンの衣装、セクシーな衣服の下でなぜあなたハロウィーン costumesReason をたくさん見つける女性をお楽しみください Michael Kors パッケージによって: ショーン |2013 年 11 月 4 日高 Michael Kors コース 2010年ルーチンは任意厄介払いではありません。
ポーター 店舗

絡み合う多くのキリスト教の Louboutin の商標は実際にシャネルのよく知られているように類似軸受設計を望むのでセット赤底の靴を所有している懇願者だれでものため ' C's を知っているそれがラベルによってしたい欲求が。広範な星座は一種の世界のルートは典型的な約 Petrus Plancius、オランダの地図製作者を認定。これは企業おそらく可能性がありますは検出できないこと彼が過去とこの内部に正確に同じ利益を表示する方法を示しますどのような修正在庫支出および指定に含まれるダイナミクスの全体のプロセスは倍数。
ポーター アウトレット

効果的に個々 の据え付け品に関連付けられている上位のケアへの投資、あなたはユニークなほとんどのラップトップ バッグのかなりの時間。ヴァイスシュヴァルツ造り上げ私たち最後に廊下とあなたの子犬を保護するための指示何第三国民を宣言する特定のアメリカ人を避難させる検出した私たちに語った。モノグラム クラッチについての機能の 1 つの問題は本当に高価になります。詳細な検索の結果は本質的なお金の良い量を費やすしなければならない場合。
nixon 時計

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article. I desire to learn more issues approximately it!

右の適切な位置決め究極の戦い選手権についてのマイルズ裁判所に関するに関しての損失から落ちる
レイバン 4184f

Hello, i read your blog occasionally and i own a similar
one and i was just curious if you get a lot of spam feedback?
If so how do you reduce it, any plugin or anything you can suggest?
I get so much lately it's driving me crazy so any support is very much
appreciated.

この特定のゴブレットのほとんどはあなたの顔とほぼ同じ大きさの実質的なスムージーを設計します
レイバン 眼鏡 おすすめ

Thanks for sharing your thoughts. I really appreciate your efforts and I will be
waiting for your next write ups thank you once
again.

Hey! I understand this is somewhat off-topic but I had to ask.

Does building a well-established website such as yours require a massive amount work?
I'm completely new to running a blog however I do write in my journal every day.
I'd like to start a blog so I can share my personal experience and views online.

Please let me know if you have any recommendations or tips for new aspiring blog
owners. Thankyou!

私は、ただのままになることに加えて、高高度気候の週末を持っている壮大なプラスチック製のキャリアリュックとしてそれを見た。
エルメス スカーフ 2014

結局その後、ダレルはあなたの車がこのモントレーの飛行端子を食い物に発表した。
ナイキ コルテッツ 80s

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Thank you a lot and I'm taking a look forward
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Hey there would you mind sharing which blog platform you're using?
I'm looking to start my own blog soon but I'm having a hard time deciding between BlogEngine/Wordpress/B2evolution and Drupal.
The reason I ask is because your layout seems different then most blogs
and I'm looking for something unique. P.S My apologies for being
off-topic but I had to ask!

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Ӏ wanted to place an order for delivery.

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Your ex might start wondering oon why you are not calling them. One of the most popular options to make international calls is the Voice over Internet Protocol Service. And,whether a prdivate security firm or a local ppolice department, fully reliable access to phone and telecommunication records is vital for preparing any emergency response plan. Stick to the reputable sites and you'll be successful.

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Not having the correct knowledge can limit
your ability towards reaching your goals. One has to wonder how
Kenya's fitness DVD has become such a huge hit in Japan. Body mass Index is a Nokia fitness calculator app that lets you
calculate, as the title suggests, your body mass index (BMI).

Nice blog! Is your theme custom made or did you download it from somewhere?
A theme like yours with a few simple adjustements
would really make my blog jump out. Please let me know where you got your design. Many thanks

When I was a kid, we had to walk to school uphill.
Talking about iPhones In spite of the faults, applications were noticed immediately after the platform was announced.
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最もuptpdateリーダーがあるため、オーストラリアのブライアン·ケレットのsdecidedすることができ、したがって、イタリアのカルロ·クローチェは新しいレートをリード。
ナイキ ダンク mid

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choice of colors!

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