Analytical Spectroscopy http://blog.lib.umn.edu/chaynes/8152/ en Copyright 2009 Wed, 25 Nov 2009 13:41:33 -0600 http://www.sixapart.com/movabletype/?v=4.25 http://blogs.law.harvard.edu/tech/rss Sample White Paper/Experimental Outline As promised, here is an example of a white paper and outline from a previous class: Download file.


]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/sample_white_paperexperimental.html http://blog.lib.umn.edu/chaynes/8152/2009/11/sample_white_paperexperimental.html Administrative Wed, 25 Nov 2009 13:41:33 -0600 Minute Papers Due 11/27/2009 Please post this week's minute papers as "comments" to this post. Minute papers should be posted by 5 pm on Friday. Feel free to read your classmate's posts.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_12082006.html http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_12082006.html Minute Papers Wed, 25 Nov 2009 10:46:00 -0600 Raman There is an article to read for the Raman spectroscopy section of class.

You should read "Raman microscopy techniques for the characterisation of pigments"by Robin J. H. Clark and coworkers before class on 11/30/09.

Discussion Questions:

1. What are the advantages and disadvantages of using Raman scattering to study important historical artifacts?

2. What is the purpose of the interference filter, the spatial filter, and the double monochromator in the common Raman instrumentation described?

3. In one described example, why are some of the white features in the text turning black? How was this revealed? Where would you find the Raman bands relevant for this discovery?

4. Can you think of other sample types where non-destructive Raman analysis would be particularly applicable?

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/raman.html http://blog.lib.umn.edu/chaynes/8152/2009/11/raman.html Papers to Read Wed, 25 Nov 2009 08:04:25 -0600 Lecture 30 Notes Download the pdf file.
Download the powerpoint file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_33_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_33_notes.html Lecture Notes Tue, 24 Nov 2009 16:26:11 -0600 Proposal Idea Discussion Just a reminder that you should come to class Wednesday with a preliminary concept for your proposal. Please keep in mind that your goal is to identify an important question/problem/hypothesis that can be investigated using an analytical spectroscopy/spectrometry technique or techniques. Please verify that the work you propose has not already been done in the literature. Please keep in mind that the project scope is 3 years.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/proposal_discussion.html http://blog.lib.umn.edu/chaynes/8152/2009/11/proposal_discussion.html Administrative Mon, 23 Nov 2009 09:50:05 -0600 Lecture 29 Notes Download the pdf file.
Download the powerpoint file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_32_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_32_notes.html Lecture Notes Sun, 22 Nov 2009 16:51:18 -0600 Luminescence There are two Analytical Chemistry A-Pages articles to read for the luminescence section of class. You should read both before class on 11/23/09

You should read "Oxygen Sensors Based on Luminescence Quenching"by James N. Demas and coworkers.

Discussion Questions for "Oxygen Sensors Based on Luminescence Quenching"

1. According to the Stern-Volmer expressions, what combination of rate constants will yield the highest sensitivity quenching sensor?

2. Why is it important that the quencher molecule have a significant Stokes shift?

3. Why is it advantageous to use a fluorophore with a long lifetime in oxygen sensor applications?

4. Come up with one experimental scenario not discussed within this paper where a luminescence-based oxygen sensor would be useful?


You should read "Analytical Bioluminescence and Chemiluminscence".

Discussion Questions for "Analytical Bioluminescence and Chemiluminescence"

1. What molecule(s) could be sensed quantitatively using a luciferin/luciferase reaction?

2. What are the advantages and disadvantages of chemiluminescence compared to fluorescence?

3. What is the difference between FRET and BRET?

4. What are the major challenges to performing in vivo spectroscopic imaging?

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/luminescence.html http://blog.lib.umn.edu/chaynes/8152/2009/11/luminescence.html Papers to Read Fri, 20 Nov 2009 08:40:16 -0600 Lecture 28 Notes Download the pdf file.
Download the powerpoint file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_31_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_31_notes.html Lecture Notes Thu, 19 Nov 2009 12:27:08 -0600 Minute Papers Due 11/20/2009 Please post this week's minute papers as "comments" to this post. Minute papers should be posted by 5 pm on Friday. Feel free to read your classmate's posts.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_12012006.html http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_12012006.html Minute Papers Thu, 19 Nov 2009 07:45:17 -0600 Lecture 27 Notes The lectures on the topic of luminescence will be given by guest lecturer Secil Koseoglu.

Download the first powerpoint file.
Download the pdf file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_30_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_30_notes.html Lecture Notes Tue, 17 Nov 2009 16:19:30 -0600 Lecture 26 Notes Download the pdf file.
Download the powerpoint file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_29_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_29_notes.html Lecture Notes Sun, 15 Nov 2009 12:12:08 -0600 Lecture 25 Notes We will continue on with the notes posted for lecture 24.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_28_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_28_notes.html Lecture Notes Thu, 12 Nov 2009 15:45:22 -0600 Minute Papers Due 11/13/2009 Please post this week's minute papers as "comments" to this post. Minute papers should be posted by 5 pm on Friday. Feel free to read your classmate's posts.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_11172006.html http://blog.lib.umn.edu/chaynes/8152/2009/11/minute_papers_due_11172006.html Minute Papers Wed, 11 Nov 2009 16:44:06 -0600 Lecture 24 Notes Download the pdf file.
Download the powerpoint file.

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_27_notes.html http://blog.lib.umn.edu/chaynes/8152/2009/11/lecture_27_notes.html Lecture Notes Tue, 10 Nov 2009 19:29:07 -0600 Infrared There is an Analytical Chemistry A-Pages articles to read for the infrared spectroscopy section of class.

You should read "IR Spectroscopy: Clinical and Diagnostic Applications" by Janie Dubois and coworkers before class on 11/13/09.

Discussion Questions for "IR Spectroscopy - Clinical and Diagnostic Applications":

1. What artifacts might be introduced while performing IR absorption measurements of biological fluids?

2. Where are the major biological IR absorption bands?

3. Is infrared absorption spectroscopy a good method to diagnose arthritis? Do infrared absorption spectroscopy diagnostics give information about the cause or progression of the disease?

4. Compare FT-IR and IR microscopy as diagnostic/clinical tools.

5. What is the diffraction limited spatial resolution in mid-IR absorption spectroscopy? Is this sufficient to probe biological tissue?

]]> http://blog.lib.umn.edu/chaynes/8152/2009/11/infrared.html http://blog.lib.umn.edu/chaynes/8152/2009/11/infrared.html Papers to Read Mon, 09 Nov 2009 14:01:46 -0600