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Kokkoli Research GroupDepartment of Chemical Engineering and Materials Science (CEMS)

Transfection Mechanisms of Polyplexes, Lipoplexes and PR_b Targeted and Non-Targeted Stealth Liposomes in α5β1 Integrin Bearing DLD-1 Colorectal Cancer Cells

Adil, M.M., Erdman, Z.S., and Kokkoli, E.

Abstract

Receptor targeted, PEGylated transfection agents can improve stability and delivery specificity of current cationic lipid and polymer based non-viral gene delivery vehicles, but their mode of transfection is poorly understood. We therefore investigated the transfection mechanisms of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)/1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) lipoplexes, branched polyethylenimine (bPEI) polyplexes, and bPEI encapsulated in either PEGylated (stealth) non-targeted liposomes or PR_b peptide (targeted to α5β1 integrin) functionalized stealth liposomes in DLD-1 colorectal cancer cells in vitro with gene expression assays, flow cytometry and confocal microscopy. DOTAP/DOPE and PR_b functionalized stealth liposomes mediated higher gene expression compared to non-targeted stealth liposomes and bPEI. However DOTAP/DOPE was internalized slowly leading to lower levels of DNA uptake. In contrast, despite high internalization of bPEI polyplexes, gene expression levels were low as DNA was unable to escape from the endosomes. Non-targeted stealth liposomes also mediated low gene expression due to low amounts of DNA internalized and slow internalization kinetics. PR_b functionalized stealth liposomes struck an optimal balance amongst these transfection agents with efficient transfection arising from fast integrin mediated internalization kinetics, high amounts of DNA uptake and endosomal escape. We found α5β1 integrin to be a valuable target for gene delivery, and that the caveolar endocytic pathway may offer an advantage to receptor targeted PEGylated transfection agents in DLD-1 cells.

Citation
Adil, M.M., Erdman, Z.S., and Kokkoli, E. "Transfection Mechanisms of Polyplexes, Lipoplexes and PR_b Targeted and Non-Targeted Stealth Liposomes in α5β1 Integrin Bearing DLD-1 Colorectal Cancer Cells ", Langmuir, 2014, 30 (13): 3802-3810.
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