August 2010 Archives

Here's a draft of two barplots that Hillary asked me to whip up using R magic. I'll let her explain what they mean.

barplots.png

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Let's take a closer look at our small, squishable friend Aphis echinaceae:


Aphid35.jpg

aren't they precious little instars?!

Aphid12.jpg

Aphid31.jpg

Aphid33.jpg

Feel free to admire this beauteous winged alate

Aphid43.jpg

This madam is so mature that she looks like Jabba the Hutt. Note the honeydew she is secreting. That bubble of delicious sugary goodness is why ants farm aphids.

There is a cool ventral shot of Jabba that the flog won't let me load up for some reason or another. Perhaps I shall try again at a later date. Until then, enjoy (it's only taken me a month to get them up)!!!

Hillary

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AphiddatasheetR.csv

I think I ought to enter a competition for who can post essentially the same data set on the flog the greatest number of times.

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AphiddatasheetRready.csv

Stuart--

Here is my aphid data ready for R tomorrow!

Hillary

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Here's the protocol for creating demoMerge.csv
Protocol for creating DemoMerge.doc

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Here's the protocol for re-finds in the remnants. Please look it over, and critique!
Protocol for seedling refinds 2010.docx

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Aphiddatasheetcorrected.csv

This is the CVS file of my aphid data after making a couple of crucial corrections. Stuart, if you would insert this into the R script that shows the graphs of the population changes on each plant, that would be wonderful!

-Hillary

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A .csv file with the list of leftover 14000 tags. These tags were not put out in 2009.

oldTags.csv

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This R script, hillaryLookAtAphids.r, allows one to view graphs of growth of aphid clones in Lauren and Hillary's experiment.

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2010 GPS Surveying Draft Protocol.pdf

Here's the GPS Surveying Protocol. Post some comments with thoughts, ideas, and changes.

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Here is our aphid data, synthesized into one large worksheet and ready to be analyzed!

Love,
Hillary (and Lauren's spirit)

Aphiddatasheet.csv

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Tag #1028 was just found in the ground near the SW corner of Hjelm house porch.

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XML is packaged for R via CRAN and is based on RSXML. Perhaps this will make it easier to parse the XML that the Topcon software puts out, or easier than trying to parse it all yourself.

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Here is a csv file of my compiled compatibility, distance, and phenology of my crosses ready to be put into R.

ian.stat.results.2010.csv

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2010 Stipa Draft Protocol.pdf. The Google Docs wasn't really working out... Comment with your thoughts and changes.

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aphidrawdata.csv

aphiddatastarted.csv

Here is our data so far. We are now working on transferring the raw data into the final form!

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1) Hillary came back, now Lauren has the other half of her brain.
2) Echinacea Team Basket Ball
3) Kate was a dinner plate for a dragonfly eating a black fly. Kinda gross, but cool.
4) Vally Fair. Josh now likes roller-coasters. Mission Accomplished.
5) Ruth Visited. Josh made cookies to celebrate.
6) Swim after work on Monday. (In our clothes... it was just that hot)
7) Finished measuring all of Kate's seedlings. They live! Mwahaha!

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Hey All,

It's been a very busy couple of weeks:

On July 27th I spoke at the Garden Club of America Zone 11 Annual Meeting in Lake Geneva. I was there for less than 48hrs, but I still had a wonderful time. I received the GCA's Fellowship in Ecological Restoration to support my Master's project, which is why I was asked to attend at all. I was pretty nervous about the speech, but in the end I think it went pretty well. They were a great audience and after this experience I feel much more confident about public speaking.

After I gave my talk, I was able go see the GCA flower show, which was really interesting. There were some pretty cool displays. I especially like the hats made of flowers, the miniatures, and the photography. They also had these challenge events where everyone was given the same supplies and theme and had a fixed amount of time to create a display. The GCA has some very creative members.

That evening we were given a tour of the lake on a boat called the Louise, where we had h'ors d'oeuvres and cocktails. The boat dropped us off at a GCA member's house for dinner. It was a lot of fun!

The next day I got to hear Kathryn Kennedy, the president of the Center for Plant Conservation, speak. She gave an excellent talk about endangered species and the challenges we face in protecting these high-risk plants in the coming decades. I was totally revved afterwords, so she did her job.

Pictures from the GCA trip:
IMG_0335.jpg

IMG_0343.JPG

IMG_0349.JPG

After I got back from Wisconsin, I had to turn around and finish working on the poster for the ESA conference. After much work, and help from Stuart, Amy, and Josh, I finally finished the poster.

I drove to St. Cloud to print both the FNC poster and Allegra's poster, and then on Sunday Stuart and I went to the ESA conference in Pittsburgh. Mimi, the REU intern from last year, was able to join us for the conference on Monday. It was great to see her again. She's currently working at Frick park as a Outdoorsy type summer camp counselor. She'll be leaving in September to teach English in Guadalupe. Should be fun!

Mimi and I got a lot of great feedback on our poster during the poster session, and I'm pretty confident that with everyone's help we can write an interesting paper.


The poster is entitled: Interspecific Co-flowering Prairie Plants Compete for Pollinators.

Here's the abstract we submitted:


Background/Question/Methods:

Pollen limitation is prevalent in many species, and can be especially worrisome in fragmented landscapes. Reproduction in the purple coneflower, Echinacea angustifolia (Asteraceae), which grows in small remnant patches of tallgrass prairie in North America, is pollen limited, but not pollinator limited.

Pollen limitation of Echinacea increases with isolation of individual plants, decreases with size of population, and has a strong negative impact on reproduction. However, pollinator visitation does not explain the reduced reproductive success. Wagenius and Lyon (2010), found that plants in the densest and largest populations of Echinacea receive fewer pollinator visits yet have greater reproductive success than plants in small isolated populations, which receive more visits but have decreased reproductive success. Among the many prairie plants pollinated by native solitary generalist bees, interspecific co-flowering plants may either enhance pollination by attracting more pollinators, or reduce reproductive success through competition or interference with compatible pollen receipt (Feldman 2008, Mitchell et al 2009).


Results/Conclusions

We investigated the community of co-flowering species surrounding a plant (floral neighborhood). We measured the effect of floral neighborhood on pollinator visitation, pollinator diversity, and pollination success of Echinacea. We randomly selected 8 focal plants at each of 10 remnant prairie sites in Douglas Co., Minnesota and observed and collected insect pollinators four times during summer 2009. We also identified and counted inflorescences of nearby co-flowering species.

We found strong evidence that floral neighborhood composition influences pollinator visitation of Echinacea. Forty species co-flowered with Echinacea, nine of which are invasive. Co-flowering species richness ranged from 14 in the largest remnant to five in the smallest. Alfalfa, Medicago sativa (Fabaceae), the most abundant exotic co-flowering species, occurred at seven of the sites, while leadplant, Amorpha canescens (Fabaceae), the most abundant native species (besides Echinacea), was only found at three remnants. The presence of alfalfa within a focal plant's floral neighborhood increased the probability of a pollinator visit by 7% (according to a glm with binomial response p<0.03). In contrast, native leadplant decreased pollinator visits by 9% (p<0.02). There is no evidence that alfalfa, leadplant, and Echinacea interact in their effect on pollinator visits. We also collected pollen from Echinacea insect pollinators and flower parts to see if patterns in floral neighborhood composition and pollinator visitation are reflected in pollen loads on pollinators or stigmas.

Here's a small copy of the actual poster:
Gallagher_ESA_Poster_Final_sm.pdf

The main conclusions were:

Neither the community of co-flowering species nor the presence of non-native plants was associated with variation in pollinator visitation.

Co-flowering species diversity was a predictor of pollinator visitation only late in the flowering season. Floral communities, as quantified by NMS analysis, were associated with the presence of non-natives all season long and overall diversity only in the early season. NMS characterizations of species co-flowering with Echinacea did not predict pollinator visitation at any time.

Echinacea neighborhoods with Amorpha, a native, had lower pollinator visitation, while the neighborhoods with Medicago, a non-native, had higher pollinator visitation.
We found no compelling evidence that interspecific co-flowering species influence pollinator visitation to Echinacea in small prairie remnants. The previously observed high pollinator visitation and concurrent pollen limitation might result from low quality of pollinator visits, an hypothesis we are now investigating.


Here's a picture of Mimi and I in front of the poster at the conference:
IMG_0353.JPG


Now that I'm back in Kensington, it's time to get back to my master's project. The work is never done! This week I'm hoping to do seedling checks for my three plots. Hope I can count on lots of help getting it done!

-Kate

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I was curious what the stigma/style looked like on psoralea and if I was actually pollinating or not. The picture displays it with pollen.Psoralea argophylla Repro400x.jpg

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Ian wants to quantify the overlap of flowering time between all pairs of plants in his experiment. This following R script reads his file with the flowering schedule of all 31 plants in his experiment and writes a file called ianPhenPairs.csv that has the flowering schedule of every possible pair combination (one per line). Note that there is a separate record for each plant as a sire and dam.

# script ian.phenology.r

pp <- read.csv("http://blog.lib.umn.edu/wage0005/echinacea/ian.phenology.final.csv")

str(pp)

p <- merge(pp,pp, by = NULL)

str(p)

31^2 == dim(p)[1]

write.csv(p, "ianPhenPairs.csv", row.names = FALSE)

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Having stickered and scanned all of the collected Stipa seed (great job Ian, Hillary, and Lauren!) we've gone through and stuck them all into their foamy rows. 1700ish seeds later, we're ready to plant out in the common garden! Here's a few pictures:

IMG_1999.jpg

sIMG_1997.jpg

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